Bovine dentin collagen/poly(lactic acid) scaffolds for teeth tissue regeneration

Abstract Electrospun scaffolds with diameter fibers compared to those in the extracellular matrix were produced poly(lactic acid) (PLA) and non-denatured collagen from bovine dentin (DCol). DCol was obtained through an improved version of Longin method by acid erosion hydroxyapatite roots teeth a 2-year-old cattle. The characterized energy dispersive X-ray spectroscopy (EDS), carbon, nitrogen, oxygen found be main elements protein. Infrared analysis revealed typical bands at about 3300, 1631, 1539, 1234 cm −1 for amides A, I, II, III, respectively. Calorimetric infrared analyses also demonstrated that non-denatured. With scanning electron microscopy, it thinnest comparable when acetic (AAc) added solution PLA trifluoroethanol (TFE). had highest porosity, we considered they could beneficial growth cell. Human placenta-derived mesenchymal stem cells seeded onto electrospun scaffolds. After 24, 48 96 h culture, cell proliferation evaluated two independent strategies. In both assays, pl-MSCs capable adhering proliferating different It observed adhesion increased significantly containing collagen, although addition AAc slightly decreased this effect on all Graphical abstract